Journal of Histochemistry and Cytochemistry Priciples for Free Access to Science
  Search:   
    >> Advanced Search

Guidelines | Subscriptions | About | exPRESS - Current - Archive | Business Information | Contact
This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by LAZARUS, S. S.
Right arrow Articles by BARDEN, H.
Right arrow Search for Related Content
PubMed
Right arrow Articles by LAZARUS, S. S.
Right arrow Articles by BARDEN, H.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?

HISTOCHEMISTRY AND ELECTRON MICROSCOPY OF MITOCHONDRIAL ADENOSINETRIPHOSPHATASE

SYDNEY S. LAZARUS 1 and HERBERT BARDEN 1

1 Isaac Albert Research Institute of the Jewish Chronic Disease Hospital, Brooklyn, New York

A study of the requirements for histochemical demonstration of mitochondrial adenosinetriphosphatase demonstrated that fixation of cryostat sections for 15 minutes in cold 0.05 M manganese chloride in 3% formol saturated with CaCO3 (pH 6.9) gave best results. By this means the enzyme could be demonstrated with both the calcium and lead incubation media. The adenosinetriphosphatase activity was demonstrated ultramicroscopically and was found to be compartmentalized in the intereristal matrix.

The histochemically demonstrated enzyme showed responses to inhibitors and activators characteristic of adenosinetriphosphatase of damaged mitochondria obtained by ultracentrifugation.

Submitted on August 7, 1961


Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:


Home page
 ScienceHome page
H. Lewis and I. Harary
Calcium-Activated Adenosine Triphosphatase Localization in Cultured Beating Heart Cells
Science, July 5, 1963; 141(3575): 47 - 48.
[Abstract] [PDF]



Guidelines | Subscriptions | About | exPRESS - Current - Archive | Business Information | Contact
The Journal of Histochemistry & Cytochemistry is owned, published, and licensed by The Histochemical Society © 1962