Journal of Histochemistry and Cytochemistry Priciples for Free Access to Science
  Search:   
    >> Advanced Search

Guidelines | Subscriptions | About | exPRESS - Current - Archive | Business Information | Contact
This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by SIEGEL, I.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by SIEGEL, I.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?

INDOPHENOL OXIDASE AND PEROXIDASE ACTIVITIES IN MAMMALIAN ERYTHROCYTES

ISRAEL SIEGEL 1

1 Zoology Department, University of Chicago, Chicago, Illinois, and Department of Microbiology, Michael Reese Hospital and Medical Center, Chicago, Illinois

Cytochrome oxidase and peroxidase activities in mammalian red cells are suggested by G- and M-Nadi reactions in rabbit, mouse, and human erythrocytes. Peroxidase activity was present in both the hemoglobin and the nonhemoglobin constituents of the red cells. Removal of oxygen stopped the G-Nadi reaction but not the M-Nadi reaction. Potassium cyanide and sodium azide at a concentration of 10–3-10–4 M partially inhibited G- and M-Nadi reactions. Microscopic examination of reacting cells revealed accumulation of reaction products at the cell periphery. This was not evident when the cells were incubated in a suspension of indophenol blue.

Mammalian erythrocytes reduced the tetrazolium salt nitro-BT. Reduction was augmented by removal of oxygen and by cytochrome oxidase inhibitors. Accumulation of formazan at the surface of reacting cells was evident upon microscopic examination.

The Nadi reactions were similar whether Ehrlich ascites tumor cells or stroma of hemolyzed erythrocytes were used. Reactions of stroma or whole erythrocytes with nitro-BT were essentially similar. Fixation or heating slowed or abolished all the reactions described.

Submitted on August 28, 1964


Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?





Guidelines | Subscriptions | About | exPRESS - Current - Archive | Business Information | Contact
The Journal of Histochemistry & Cytochemistry is owned, published, and licensed by The Histochemical Society © 1965