Journal of Histochemistry and Cytochemistry Priciples for Free Access to Science
  Search:   
    >> Advanced Search

Guidelines | Subscriptions | About | exPRESS - Current - Archive | Business Information | Contact
This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by FROST, J. L.
Right arrow Articles by BRANDES, D.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by FROST, J. L.
Right arrow Articles by BRANDES, D.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?

NONSPECIFIC ESTERASES IN RAT PROSTATIC EPITHELIAL CELLS

JAMES L. FROST 1 and DAVID BRANDES 1

1 Departments of Pathology, The Johns Hopkins University School of Medicine and The Baltimore City Hospitals, Baltimore, Maryland

Prostates from normal and recently castrated rats were examined for the localization of esterase activity using Holt's indoxyl acetate method. Tissue fixed in formalin mixtures containing sucrose showed preservation of abundant cytoplasmic esterase activity while tissue fixed in formalin mixtures lacking sucrose had little cytoplasmic esterase activity. Esterase activity in droplets was abundant after both fixations. The difference could not be explained by ferroferricyanide inhibition, pH or osmolality of the fixative, and is attributed to a protective effect of sucrose during fixation. E600 greatly reduced cytoplasmic esterase activity in normal and castrated rats. Esterase activity in droplets was resistant to E600 in both normals and castrates. Ribonuclease extraction reduced cytoplasmic esterase activity in both normal and castrated rats only slightly, indicating no association of cytoplasmic esterases with ribosomal ribonucleic acid. Cytoplasmic esterase activity is reduced in castrates in which the epithelial cells of the prostate also show a decrease in endoplasmic reticulum and ribosomes. Similarity of location of droplet esterase activity and of lysosomes marked by acid phosphatase and reduction in droplet esterase activity by saline extraction are cited as evidence for a lysosomal localization of E600-resistant esterase.

Submitted on April 5, 1967


Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?





Guidelines | Subscriptions | About | exPRESS - Current - Archive | Business Information | Contact
The Journal of Histochemistry & Cytochemistry is owned, published, and licensed by The Histochemical Society © 1967