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A THIOCHOLINE-LEAD FERROCYANIDE METHOD FOR ACETYLCHOLINESTERASE

OLAVI ERANKÖ 1, GEORGE B. KOELLE 1, and LIISA RÄISÄNEN 1

1 Department of Pharmacology, Medical School, University of Pennsylvania, Philadelphia, Pennsylvania

The principle employed in the thiocholine-copper ferrocyanide method of Karnovsky and Roots has been adopted for the development of a similar procedure in which lead, complexed with Tris-acetate buffer, is used as the trapping agent for the ferrocyanide ion, formed by the preferential reduction of ferricyanide by thiocholine released enzymatically from acetylthiocholine. The resulting colloidal, faintly yellowish white precipitate, Pb2Fe(CN)6, can be viewed directly by light or phase contrast microscopy, or more easily following its conversion to PbS. The method has been shown to produce extremely fine localization of acetylcholinesterase in the cat superior cervical and stellate ganglia and at the motor end plates of mouse intercostal muscle.

Submitted on May 25, 1967


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J. S. Hanker, W. A. Anderson, and F. E. Bloom
Osmiophilic Polymer Generation: Catalysis by Transition Metal Compounds in Ultrastructural Cytochemistry
Science, March 3, 1972; 175(4025): 991 - 993.
[Abstract] [PDF]



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