ON THE HISTOCHEMISTRY OF AZO GROUP-FREE THIAZOLE DYES
1 Department of Pathology, Warren State Hospital, Warren, Pennsylvania
Analysis of primuline, thioflavine S and thioflavine T acid and basic azo group-free thiazole dyes showed that they were built up from a number of components which were characterized by physicochemical methods. It was found that the isolated components, as well as related substances of known chemical composition, have characteristic staining properties. Factors involved in the staining mechanism of the dyes and of componentsdye concentration, pH, aggregation of the dye moleculeswere investigated and their roles are discussed. Selectivity of these fluorescent staining methods was also studied. Submitted on March 14, 1966
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