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FREEZE-DRYING OF SMALL TISSUE SAMPLES AND THIN FROZEN SECTIONS BELOW –60°C A SIMPLE METHOD OF CRYOSORPTION PUMPING

WALTER E. STUMPF 1 and LLOYD J. ROTH 1

1 Department of Pharmacology, University of Chicago, Chicago, Illinois

Freeze-drying rates for three tissues have been determined by direct measurement of weight loss in a closed system, using an electrobalance. The half-drying times (T/2) at approximately 1 x 10–5 mm Hg with Dry Ice slush as coolant were determined for brain, kidney and liver and found to be 100, 50 and 30 hr, respectively, for 10-mg samples of tissue. A method for the freeze-drying of frozen sections in which the temperature of the tissue is maintained below –60°C throughout sectioning and drying is discussed. A simple portable, all glass, freeze-drying system utilizing cryosorption pumping is presented.

Submitted on August 3, 1966


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I. M. Diab, D. X. Freedman, and L. J. Roth
[3H]Lysergic Acid Diethylamide:Cellular Autoradiographic Localization in Rat Brain
Science, September 10, 1971; 173(4001): 1022 - 1024.
[Abstract] [PDF]



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