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A COMBINED PROCEDURE FOR THE HISTOCHEMICAL FLUORESCENCE DEMONSTRATION OF MONOAMINES AND MICROAUTORADIOGRAPHY OF WATER-SOLUBLE DRUGS

DAVID MASUOKA 1 and GIAN-FRANCO PLACIDI 1

1 Psychopharmacology Research Laboratories, Veterans Administration Hospital, Sepulveda, California, and Department of Pharmacology, University of California, Irvine-California College of Medicine, Los Angeles, California

A procedure for combining microautoradiography and the histochemical fluorescence method for aromatic monoamines on the same tissue section is described. The use of aqueous solutions is completely avoided, thus eliminating artifacts due to diffusion and dislocation of the labeled water-soluble drug and endogenous amines. The combination of these two techniques provides a means for obtaining data hitherto unavailable to correlate the site of binding and/or storage of water-soluble drugs with monoaminergic nerve cells and terminals.

Submitted on May 6, 1968


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