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CHOLINESTERASES AND NONSPECIFIC ESTERASES OF DEVELOPING AND ADULT (NORMAL AND ATROPHIC) RAT GASTROCNEMIUS 1. CHEMICAL ASSAY AND ELECTROPHORESIS

KEVIN D. BARRON 1, A. T. ORDINARIO 1, J. BERNSOHN 1, A. R. HESS 1, and M. T. HEDRICK 1

1 Neuropathology Research Section and the Neuropsychiatry Research Laboratory, Veterans Administration, Hines, Illinois, and the Department of Neurology and Psychiatry, Northwestern University Medical School, Chicago, Illinois

Cholinesterases and nonspecific esterases of normal and atrophic (denervated and tenotomized) adult rat gastrocnemius were assayed, the latter 5-45 days after operation. Acetylcholinesterase activity per whole muscle was about 25% of normal 5 days after denervation and, in permanently denervated animals, showed little change thereafter. Tenotomy caused a transient 27% fall. Butyrylcholinesterase was unaffected. Nonspecific esterase activity of denervated gastrocnemius (substrate, agr-naphthyl acetate) was normal 30 days after reversible sciatic injury and 50% of normal 45 days after irreversible denervation. Wet weight and total protein fell in approximate parallel. Results of assay and electrophoresis (zymograms) showed that muscle esterase was mainly of B type, although A and C type acetylesterases accounted for about 10% of the free or soluble fraction. An increase in the free esterase activity of the homogenate and an absolute increase in A type esterase accompanied denervation and disuse. Bound esterase was 80% released by Triton X-100 and was entirely B type. Zymograms of neonatal muscle were distinguished by the prominence of A-esterases and the presence of an acetylcholinesterase which was not encountered in adults. Over-all, despite some points of similarity, zymograms of neonatal and atrophic adult muscles were distinctly different.

Submitted on January 26, 1968


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