Journal of Histochemistry and Cytochemistry Priciples for Free Access to Science
  Search:   
    >> Advanced Search

Guidelines | Subscriptions | About | exPRESS - Current - Archive | Business Information | Contact
This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by GALJAARD, H.
Right arrow Articles by GIESEN, J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by GALJAARD, H.
Right arrow Articles by GIESEN, J.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?

A QUANTITATIVE HISTOCHEMICAL STUDY OF INTESTINAL MUCOSA AFTER X-IRRADIATION

H. GALJAARD 1, J. BUYS 1, M. VAN DUUREN 1, and J. GIESEN 1

1 Medical Biological Laboratory of the National Defence Organisation TNO, Rijswijk (Z.H.), The Netherlands

The effect of low doses of x-irradiation (50-400 R) on the activity of various enzymes in rat intestinal epithelium has been investigated by histochemical staining methods and quantitative microchemical analyses of crypts and villi dissected from frozen-dried sections. Irradiation had no effect on the activities of enzymes which in nonirradiated animals are present exclusively or mainly in the villus epithelium (aminopeptidase, various phosphatases) or are evenly distributed along the epithelium of crypts and villi (various dehydrogenases). However, nonspecific esterase activity decreased markedly both in crypt epithelium and villus epithelium. This occurred 36 hr after irradiation, independent of the radiation dose. The number of crypt cells with reduced esterase activity and the duration of the effect increased with higher radiation doses. These results were confirmed by quantitative analyses which also showed that esterase activity is 5 times higher in the villus than in the crypt. The remarkable correspondance between the period of reduced esterase activity in the crypt and the period of increased proliferative activity after various radiation doses suggest a relationship between changes in crypt cell population dynamics and esterase activity; the functional consequences for the villus epithelium of changes in the crypt cells after irradiation are discussed.

Submitted on July 20, 1969


Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?





Guidelines | Subscriptions | About | exPRESS - Current - Archive | Business Information | Contact
The Journal of Histochemistry & Cytochemistry is owned, published, and licensed by The Histochemical Society © 1970