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MICROPROBE ANALYSIS OF PROTARGOL STAIN DEPOSITION IN TWO PROTOZOA

IAN S. ZAGON 1, JIRI VAVRA 1, and IAN STEELE 1

1 Department of Zoology, University of Illinois, Urbana, Illinois 61801, and University of Colorado Medical Center, Denver, Colorado 80220, College of Veterinary Medicine, University of Illinois, Urbana, Illinois 61801, and Institute of Parasitology, Czechoslovak Academy of Science, Prague, Czechoslovakia, and Department of Geology, University of Illinois, Urbana, Illinois 61801

Protargol-stained specimens of two protozoans, Carchesium polypinum (Ciliata, Peritrichida) and Gregarina rhyparobiae (Sporozoa, Eugregarinida), were examined by an electron microprobe analyzer in order to determine the elemental composition of the stain deposit. Limited penetration of the microprobe beam presumably revealed only the composition of the stain deposits close to the surface of the examined protozoa. The data on this first analysis of Protargol staining revealed that element detection was negligible in the unstained preparations, but the stained specimens of both protozoans revealed a mass concentration ratio of 0.4 Cu:1 Ag:6.1 Au. There was no significant difference in the element ratio between the two protozoan species belonging to different classes and stained with slightly different stain procedures. Some aspects of Protargol mechanisms are discussed along with an interpretation of data and an indication of the feasibility of further examinations.

Submitted on February 27, 1970


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