|
|
|
|
 |
|||
|
|||
Spectral studies on 33258 Hoechst and related bisbenzimidazole dyes useful for fluorescent detection of deoxyribonucleic acid synthesis
SA Latt and G Stetten
Absorption, fluroescence and circular dichroism measrements on 33258 Hoechst-deoxyribonucleic acid (DNA) complexes are consistent with the existence of two types of dye-binding interactions. One type, which persists at elevated solution ionic strength, is highly specific for adenine-thymine-rich DNA. Dye bound under this condition exhibits efficient fluorescence and strong optical activity. A less specific, largely electrostatic interaction is associated with less intense fluorescence and weaker optical activity. The fluorescence of 33258 Hoechst and several other bisbenzimidazole dyes is less when bound to poly(deoxyadenylate-5-bromodeoxyuridylate) than when bound to poly(deoxyadenlyate-deoxythymidylate). Quenching of 33258 Hoechst fluorescence can also be used to detect biosynthetic incorporation of 5- bromodeoxyuridine into the DNA of living cells. This property of 33258 Hoechst should allow fluorescence-activated cell and chromosome sorting according to the extent of DNA synthesis, providing a bridge between biochemical and cytologic analyses of processes related to DNA replication.
Volume 24,
Issue 1,
pp. 24-33,
01/01/1976
Copyright © 1976 by The Histochemical Society
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  H. Zipper, H. Brunner, J. Bernhagen, and F. Vitzthum Investigations on DNA intercalation and surface binding by SYBR Green I, its structure determination and methodological implications Nucleic Acids Res., July 12, 2004; 32(12): e103 - e103. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. A. Miyakawa, M. de Lourdes Junqueira, and J. E. Krieger Identification of two novel shear stress responsive elements in rat angiotensin I converting enzyme promoter Physiol Genomics, April 13, 2004; 17(2): 107 - 113. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. K. Pal, L. Zhao, and A. H. Zewail Water at DNA surfaces: Ultrafast dynamics in minor groove recognition PNAS, July 8, 2003; 100(14): 8113 - 8118. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. D. Bradshaw, D. C. Graves, K. Motamed, and E. H. Sage SPARC-null mice exhibit increased adiposity without significant differences in overall body weight PNAS, May 13, 2003; 100(10): 6045 - 6050. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Kaidi, S. Bernard, P. Lambert, A. Massip, F. Dessy, and I. Donnay Effect of Conventional Controlled-Rate Freezing and Vitrification on Morphology and Metabolism of Bovine Blastocysts Produced In Vitro Biol Reprod, October 1, 2001; 65(4): 1127 - 1134. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. Neuber and R.D. Powers Is the mouse a clinically relevant model for human fertilization failures? Hum. Reprod., January 1, 2000; 15(1): 171 - 174. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. C. Stockert, C. I. Trigoso, T. Cuellar, J. L. Bella, and J. A. Lisanti A New Fluorescence Reaction in DNA Cytochemistry: Microscopic and Spectroscopic Studies on the Aromatic Diamidino Compound M&B 938 J. Histochem. Cytochem., January 1, 1997; 45(1): 97 - 106. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. van de Sande, N. Ramsing, M. Germann, W Elhorst, B. Kalisch, E von Kitzing, R. Pon, R. Clegg, and T. Jovin Parallel stranded DNA Science, July 29, 1988; 241(4865): 551 - 557. [Abstract] [PDF]
|
|
| Guidelines | Subscriptions | About | exPRESS - Current - Archive | Business Information | Contact |