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A high efficiency flow cytometer

MJ Skogen-Hagenson, GC Salzman, PF Mullaney and WH Brockman

A flow chamber has been developed which collects about 60% of the total cell fluorescence for analysis compared to about 2.5% for conventional flow systems. The chamber, an ellipsoid of revolution, is gold-plated for increased reflectivity. Fluorochrome-stained cells enter the flow cell directly above the primary focus of the ellipsoid at the rate of 1000 cell/sec. A focused argon-ion laser beam enters the flow cell parallel to the semiminor axis and intersects the cell stream at the primary focus. Fluorescent light emanating from this point is reflected toward the secondary focus, where it exits the chamber for analysis. The high efficiency flow cytometer has been used to obtain nucleotide fluorescence distributions from samples of Micrococcus glutamicus bacteria stained with propidium iodide and of spermatozoa stained by the acriflavine-Feulgen procedure.

Volume 25, Issue 7, pp. 784-789, 07/01/1977
Copyright © 1977 by The Histochemical Society


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P. Horan and L. Wheeless Jr
Quantitative single cell analysis and sorting
Science, October 14, 1977; 198(4313): 149 - 157.
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