|
|
|
|
 |
|||
|
|||
Rapid hypotonic method for flow cytofluorometry of monolayer cell cultures. Some pitfalls in staining and data analysis
J Fried, AG Perez and BD Clarkson
The rapid hypotonic staining procedure developed by Krishan for DNA determinations by flow cytofluorometry has been proven accurate for in vivo cell samples and for cell lines growing in suspension culture. We show that the unmodified procedure may produce distorted DNA histograms when used for staining cells growing in monolayer cultures, however. To eliminate these distortions, it was necessary to avoid the use of trypsin by staining the attached cells directly, using a hypotonic fluorochrome solution to which nonionic detergent was added. Two sublines of HeLa S3 cells are shown to exhibit major differences in their staining characteristics. By using our revised staining procedure, the two sublines appear to produce very satisfactory DNA histograms. However, in only one subline does the S phase fraction calculated from the histograms agree with the autoradiographical labeling index. Mitotic cells remain intact under these staining conditions, and the principal observed effect of nonionic detergents in this case is to decrease the coefficient of variation of fluorescence intensity.
Volume 26,
Issue 11,
pp. 921-933,
11/01/1978
Copyright © 1978 by The Histochemical Society
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  A. J. Stel, B. ten Cate, S. Jacobs, J. W. Kok, D. C. J. Spierings, M. Dondorff, W. Helfrich, H. C. Kluin-Nelemans, L. F. M. H. de Leij, S. Withoff, et al. Fas Receptor Clustering and Involvement of the Death Receptor Pathway in Rituximab-Mediated Apoptosis with Concomitant Sensitization of Lymphoma B Cells to Fas-Induced Apoptosis J. Immunol., February 15, 2007; 178(4): 2287 - 2295. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. R. Smith, F. Jin, and I. Joshi Enhanced efficacy of therapy with antisense BCL-2 oligonucleotides plus anti-CD20 monoclonal antibody in scid mouse/human lymphoma xenografts Mol. Cancer Ther., December 1, 2004; 3(12): 1693 - 1699. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. F. Kuemmerle, H. Zhou, and J. G. Bowers IGF-I stimulates human intestinal smooth muscle cell growth by regulation of G1 phase cell cycle proteins Am J Physiol Gastrointest Liver Physiol, March 1, 2004; 286(3): G412 - G419. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 I. M. Pedersen, A. M. Buhl, P. Klausen, C. H. Geisler, and J. Jurlander The chimeric anti-CD20 antibody rituximab induces apoptosis in B-cell chronic lymphocytic leukemia cells through a p38 mitogen activated protein-kinase-dependent mechanism Blood, February 15, 2002; 99(4): 1314 - 1319. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. J. Meszaros, J. S. Reichner, and J. E. Albina Macrophage-Induced Neutrophil Apoptosis J. Immunol., July 1, 2000; 165(1): 435 - 441. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. A. Pahlevan, D. J. M. Wright, C. Andrews, K. M. George, P. L. C. Small, and B. M. Foxwell The Inhibitory Action of Mycobacterium ulcerans Soluble Factor on Monocyte/T Cell Cytokine Production and NF-{kappa}B Function J. Immunol., October 1, 1999; 163(7): 3928 - 3935. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. H. Holmstrom, S. E. F. Tran, V. L. Johnson, N. G. Ahn, S. C. Chow, and J. E. Eriksson Inhibition of Mitogen-Activated Kinase Signaling Sensitizes HeLa Cells to Fas Receptor-Mediated Apoptosis Mol. Cell. Biol., September 1, 1999; 19(9): 5991 - 6002. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Shan, O. W. Press, T. T. Tsu, M. S. Hayden, and J. A. Ledbetter Characterization of scFv-Ig Constructs Generated from the Anti-CD20 mAb 1F5 Using Linker Peptides of Varying Lengths J. Immunol., June 1, 1999; 162(11): 6589 - 6595. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Ju, J. Pedersen-Lane, F. Maley, and E. Chu Regulation of p53 expression by thymidylate synthase PNAS, March 30, 1999; 96(7): 3769 - 3774. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. Chu, S. M. Copur, J. Ju, T.-m. Chen, S. Khleif, D. M. Voeller, N. Mizunuma, M. Patel, G. F. Maley, F. Maley, et al. Thymidylate Synthase Protein and p53 mRNA Form an In Vivo Ribonucleoprotein Complex Mol. Cell. Biol., February 1, 1999; 19(2): 1582 - 1594. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Yada, K. Kishihara, Y.-Y. Kong, and K. Nomoto Differential Requirements of CD45 Protein Tyrosine Phosphatase for Cytolytic Activities and Intrathymic and Extrathymic Development of Intestinal Intraepithelial Lymphocytes J. Immunol., September 1, 1998; 161(5): 2208 - 2216. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Shan, J. A. Ledbetter, and O. W. Press Apoptosis of Malignant Human B Cells by Ligation of CD20 With Monoclonal Antibodies Blood, March 1, 1998; 91(5): 1644 - 1652. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. W. Lee, C. Beckham, B. R. Michel, H. Rosen, and H. J. Deeg HLA-DR-Mediated Signals for Hematopoiesis and Induction of Apoptosis Involve But Are Not Limited to a Nitric Oxide Pathway Blood, July 1, 1997; 90(1): 217 - 225. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. Rein, D. A. Natale, U. Gartner, M. Niggemann, M. L. DePamphilis, and H. Zorbas Absence of an Unusual "Densely Methylated Island" at the Hamster dhfr ori-beta J. Biol. Chem., April 11, 1997; 272(15): 10021 - 10029. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. E. F. Tran, T. H. Holmstrom, M. Ahonen, V.-M. Kahari, and J. E. Eriksson MAPK/ERK Overrides the Apoptotic Signaling from Fas, TNF, and TRAIL Receptors J. Biol. Chem., May 4, 2001; 276(19): 16484 - 16490. [Abstract] [Full Text] [PDF]
|
|
| Guidelines | Subscriptions | About | exPRESS - Current - Archive | Business Information | Contact |