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Detection of tubulin and actin in various cell lines by an immunoperoxidase technique

E Karsenti, B Guilbert, M Bornens, S Avrameas, R Whalen and D Pantaloni

This paper reports on the preparation of immunsera against tubulin and actin, and the purification of anti-tubulin and anti-actin antibodies on immunoadsorbent columns. These purified antibodies were used in an indirect immunoperoxidase assay to visualize microtubules and microfilaments in various cell lines. The specificity of antibodies and the methods of cell fixation required are discussed, as well as some aspects of microtubule and microfilament organization, as visualized by this technique.

Volume 26, Issue 11, pp. 934-947, 11/01/1978
Copyright © 1978 by The Histochemical Society


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