|
|
|
|
 |
|||
|
|||
The effect of aldehyde fixation on selected substrates for energy metabolism and amino acids in mouse brain
KA Conger, JH Garcia, AS Lossinsky and FC Kauffman
The effect of aldehyde fixation on concentrations of low molecular weight constituents was determined by comparing amounts of selected intermediates in brains of mice exposed to aldehyde fixative solutions with those perfused with phosphate buffer solution alone. Aldehyde perfusion resulted in excellent preservation of cerebral cortex ultrastructure in the presence of dramatic declines in adenosine triphosphate, phosphocreatine, glucose and glucose-6-phosphate that occureed before exposure of the tissue to aldehyde fixatives. Decreases in hexose were accompanied by approximately a 4-fold increase in lactate and a 2-fold increase in pyruvate. Glycogen levels decreased by about 60% during the initial operative procedure but remained constant after aldehyde fixation. Glycogen content declined approximately 90% in tissues that were not treated with aldehyde. Concentrations of aspartate and glutamate changed only slightly during the initial period (1-5 min) and remained constant for at least 90 min in cerebral cortices fixed with aldehydes. Alanine levels increased in both fixed and unfixed tissue; however, this increase was much smaller in tissues exposed promptly to aldehydes. Total ninhydrin-positive material in perchloric acid extracts of brain decreased in mice exposed to aldehyde solutions but increased in tissues that were not. These results indicated that several amino acids may be measured reliably in tissues preserved for light and electron microscopy. In addition, determination of glutamate: alanine ratios in tissues perfused with aldehydes may provide an indication of the timing of fixation.
Volume 26,
Issue 6,
pp. 423-433,
06/01/1978
Copyright © 1978 by The Histochemical Society
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  M. Caputo, W.C. Dihmis, A.J. Bryan, M.-S. Suleiman, and G.D. Angelini Warm blood hyperkalaemic reperfusion (`hot shot') prevents myocardial substrate derangement in patients undergoing coronary artery bypass surgery Eur. J. Cardiothorac. Surg., May 1, 1999; 13(5): 559 - 564. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Caputo, A.J. Bryan, A.M. Calafiore, M.-S. Suleiman, and G.D. Angelini Intermittent antegrade hyperkalaemic warm blood cardioplegia supplemented with magnesium prevents myocardial substrate derangement in patients undergoing coronary artery bypass surgery Eur. J. Cardiothorac. Surg., December 1, 1998; 14(6): 596 - 601. [Abstract] [Full Text] [PDF]
|
|
| Guidelines | Subscriptions | About | exPRESS - Current - Archive | Business Information | Contact |