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Inferences on RNA synthesis rates during mitosis from flow cytofluorimetric RNA histograms

JV Watson and SH Chambers

Flow cytofluorimetric techniques with acridine orange staining have been used to study RNA levels of cells during and immediately after mitosis. It can be inferred from the histogram data that, of the mitotic cells, those in telophase have the highest RNA content, and that the rate of synthesis increases rapidly between anaphase and telophase. These inferences correlate with results obtained from parallel labeled precursor studies.

Volume 26, Issue 9, pp. 691-695, 09/01/1978
Copyright © 1978 by The Histochemical Society


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