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Cellular autofluorescence--is it due to flavins?

RC Benson, RA Meyer, ME Zaruba and GM McKhann

For many cell types, cellular autofluorescence in the 500-600 nm spectral region can result in a significant background signal for measurements of weakly fluorescent probes. Measurements of fluorescence intensity and spectra of a variety of cell types and assays of neuronal homogenates are presented to demonstrate that this autofluorescence is most likely due to endogenous flavoproteins.

Volume 27, Issue 1, pp. 44-48, 01/01/1979
Copyright © 1979 by The Histochemical Society


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