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The use of the potassium pyroantimonate-osmium method as a means of identifying and localizing calcium at the ultrastructural level in the cells of calcifying systems

J Appleton and DC Morris

Potassium pyroantimonate-osmium has been used to localize calcium as an electron dense precipitate in the odontoblast, ameloblast and early hypertrophic chondrocytes of the mandibular condylar growth cartilage. The precipitate exhibited a specific association with various cell organelles. The presence of calcium in the precipitates was confirmed using energy dispersive analysis by x-rays. It is suggested that this K- pyroantimonate method provides an accurate technique for the investigation of the subcellular localization of calcium in calcifying systems.

Volume 27, Issue 2, pp. 676-680, 02/01/1979
Copyright © 1979 by The Histochemical Society


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