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Demonstration of alpha 1-antitrypsin by immunofluorescence on paraffin- embedded hepatic and pancreatic tissue

MJ McElrath, RM Galbraith and RC Allen

Studies were performed in an attempt to improve current immunohistological techniques for the demonstration of alpha 1- antitrypsin (A1AT) in formalin-fixed paraffin-embedded tissues. The unwanted fluorescence (UF) commonly occurring in such procedures was found to be effectively eliminated by immunoadsorption of A1AT antisera with human serum lacking A1AT (Pi-null phenotype) coupled in solid phase to glutaraldehyde-activated aminohexyl-Sepharose 4B. Specificity of the antisera for A1AT was established by subsequent solid phase immunoadsorption against normal human serum bound to AH-Sepharose 4B. Using these techniques, immunoreactive A1AT was demonstrated in the cytoplasm of hepatocytes in liver biopsies obtained from patients with Z and MZ serum phenotypes, and in the cytoplasm of normal pancreatic islet cells.

Volume 27, Issue 3, pp. 794-796, 03/01/1979
Copyright © 1979 by The Histochemical Society


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