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Complement-mediated binding of immunoglobulins to some endocrine cells of the pancreas and gut

R Buffa, E Solcia, R Fiocca, O Crivelli and A Pera

Unspecific binding of immunoglobulins to gastrin, somatostain and glucagon cells of the gastrointestinal mucosa or pancreas has been found to occur through a nonantigen-antibody mechanism mediated by the C14 fraction of complement. The phenomenon represents an important drawback in hormone immunohistochemistry, which can be overcome by using complement deprived, highly dilute anti-hormone sera.

Volume 27, Issue 9, pp. 1279-1280, 09/01/1979
Copyright © 1979 by The Histochemical Society


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The Journal of Histochemistry & Cytochemistry is owned, published, and licensed by The Histochemical Society © 1979