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A rapid method for embedding tissues for electron microscopy using 1,4- dioxane and Polybed 812

TP Shearer and LG Hunsicker

A rapid method for embedding tissues for electron microscopy is described. This method, which can be completed within 5 hr, uses 1,4- dioxane as the final dehydrating agent and Polybed 812 as the embedding medium. Satisfactory preservation of cellular structures is consistently achieved with a variety of normal and diseased tissues. This method may be of particular value to diagnostic electron microscopy laboratories where time and simplicity are critical.

Volume 28, Issue 5, pp. 465-467, 05/01/1980
Copyright © 1980 by The Histochemical Society


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The Journal of Histochemistry & Cytochemistry is owned, published, and licensed by The Histochemical Society © 1980