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Suppression of endogenous avidin-binding activity in tissues and its relevance to biotin-avidin detection systems

GS Wood and R Warnke

As biotin-avidin systems continue to be developed for applications involving single cells, cell suspensions, and especially tissue sections, the need arises for a method of blocking endogenous avidin- binding activity. One such method is described and its proposed mechanism is discussed. Utilizing this method, endogenous avidin- binding activity was detected and suppressed in selected human and murine tissues, thus facilitating the interpretation of specific immunohistochemical staining utilizing hybridoma monoclonal antibodies in a biotin-avidin-horseradish peroxidase detection system.

Volume 29, Issue 10, pp. 1196-1204, 10/01/1981
Copyright © 1981 by The Histochemical Society


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