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PROBLEMS IN QUANTITATING RENAL ENZYMES

J. B. LONGLEY PH.D.1 and R. C. BAHN M.D.1

1 Laboratory of Pathology, and Histochemistry, NIAMD, National Institutes of Health, Bethesda 14, Maryland

Histochemical assay methods for renal dehydrogenase present both biochemical and morphological problems. In each case practical solutions for these problems have been found. By a combination of simple procedures it is possible to derive an estimate of succinic dehydrogenase activity of the major reactive histological components of the kidney. The general approach should be useful in the future study of the response of renal dehydrogenase to experimental procedures.

Submitted on April 29, 1955


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