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THE HISTOCHEMICAL DEMONSTRATION OF ACID PHOSPHATASE BY A POST-INCUBATION COUPLING TECHNIQUE

ALEXANDER M. RUTENBURG 1 and ARNOLD M. SELIGMAN 1

1 Yamins Laboratory for Surgical Research, Beth Israel Hospital, the Departments of Surgery, Harvard Medical School, Sinai Hospital of Baltimore and The Johns Hopkins University School of Medicine

The procedure for the histochemical demonstration of acid phosphatase by a post-incubation coupling technique using sodium 6-benzoyl-2-naphthyl phosphate is described. The distribution of activity in the tissues of the rat is presented. Enzymatic activity was generally confined to the cytoplasm of epithelial cells. Prostate, liver, spleen, ovary and kidney showed the greatest acid phosphatase activity.

Submitted on February 1, 1955


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