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PITFALLS IN HISTOCHEMISTRY: AN UNSUCCESSFUL ATTEMPT TO DEMONSTRATE MUCOID SULFATASE ACTIVITY IN MAMMALIAN TISSUE

CLAES-HENRIK DOHLMAN M.D.1 and JONAS S. FRIEDENWALD M.D.1

1 Wilmer Ophthalmological Institute of the Johns Hopkins University and Hospital

Attempts to demonstrate a sulfatase in mammalian tissues attacking sulfated mucopolysaccharides were unsuccessful. Both chemical and histochemical methods were employed.

Some risks involved in the use of the lead salt technique in enzyme histochemistry are pointed out.

Submitted on April 7, 1955


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