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Aluminum phosphate visualisation of acid phosphatase activity: a biochemical and x-ray microanalysis study

JP Berry, J Hourdry, M Sternberg and P Galle

A new method is described that demonstrates acid phosphatase activity in the cells of the proximal tubules of the rat kidney. The method is based on the formation of an insoluble aluminum phosphate precipitate. Microanalysis was used to demonstrate the presence of intracellular aluminum and determine the quantity present under the probe. Parallel biochemical studies showed that the aluminum precipitate was indeed due to acid phosphatase activity.

Volume 30, Issue 1, pp. 86-90, 01/01/1982
Copyright © 1982 by The Histochemical Society


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Home page
 Hum Exp ToxicolHome page
J.P. Berry, R. Masse, F. Escaig, and P. Galle
Intracellular Localization of Cerium. A Microanalytical Study using an Electron Microprobe and Ionic Microanalysis
Human and Experimental Toxicology, November 1, 1989; 8(6): 511 - 520.
[Abstract] [PDF]



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