Journal of Histochemistry and Cytochemistry Priciples for Free Access to Science
  Search:   
    >> Advanced Search

Guidelines | Subscriptions | About | exPRESS - Current - Archive | Business Information | Contact
This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Hsu, S. M.
Right arrow Articles by Soban, E.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Hsu, S. M.
Right arrow Articles by Soban, E.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?

Color modification of diaminobenzidine (DAB) precipitation by metallic ions and its application for double immunohistochemistry

SM Hsu and E Soban

Three metallic ions, NiCl2, CoCl2, and CuSO4, were found to modify the color of the normally brown diaminobenzidine (DAB) reaction. The colors ranged from purplish blue (NiCl2), dark blue/bluish black (CoCl2), to greyish blue (CuSO4). We have found that the CoCl2 + DAB is the ion of choice because: 1) it yields a distinct dark blue color that is easily distinguishable from brown DAB; 2) the blue reaction product is very stable throughout the entire staining procedure; and 3) background staining is minimal. These findings can be applied to the double staining technique of two different antigens in the same section. Among three staining procedures discussed, the avidin-biotin peroxidase complex (Co-DAB)-peroxidase-antiperoxidase (DAB) technique produced the best results because: 1) no antibody elution was needed following the avidin-biotin-peroxidase complex procedure when the CoCl2-DAB modification was used; and 2) no background staining occurred.

Volume 30, Issue 10, pp. 1079-1082, 10/01/1982
Copyright © 1982 by The Histochemical Society


Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:


Home page
 J. Bacteriol.Home page
S. Aygun-Sunar, R. Bilaloglu, H. Goldfine, and F. Daldal
Rhodobacter capsulatus OlsA Is a Bifunctional Enyzme Active in both Ornithine Lipid and Phosphatidic Acid Biosynthesis
J. Bacteriol., December 1, 2007; 189(23): 8564 - 8574.
[Abstract] [Full Text] [PDF]

Home page
 Cancer Res.Home page
J. Villar, M. I. Arenas, C. M. MacCarthy, M. J. Blanquez, O. M. Tirado, and V. Notario
PCPH/ENTPD5 Expression Enhances the Invasiveness of Human Prostate Cancer Cells by a Protein Kinase C{delta} Dependent Mechanism
Cancer Res., November 15, 2007; 67(22): 10859 - 10868.
[Abstract] [Full Text] [PDF]

Home page
 Ann OncolHome page
I. Conde, R. Paniagua, J. Zamora, M. J. Blanquez, B. Fraile, A. Ruiz, and M. I. Arenas
Influence of thyroid hormone receptors on breast cancer cell proliferation
Ann. Onc., January 1, 2006; 17(1): 60 - 64.
[Abstract] [Full Text] [PDF]

Home page
 J. Clin. Pathol.Home page
T Krenacs, E Bagdi, E Stelkovics, L Bereczki, and L Krenacs
How we process trephine biopsy specimens: epoxy resin embedded bone marrow biopsies
J. Clin. Pathol., September 1, 2005; 58(9): 897 - 903.
[Abstract] [Full Text] [PDF]

Home page
 J. Histochem. Cytochem.Home page
H.-G. Bernstein, C. I. Seidenbecher, K.-H. Smalla, E. D. Gundelfinger, B. Bogerts, and M. R. Kreutz
Distribution and Cellular Localization of Caldendrin Immunoreactivity in Adult Human Forebrain
J. Histochem. Cytochem., August 1, 2003; 51(8): 1109 - 1112.
[Abstract] [Full Text] [PDF]

Home page
 J AndrolHome page
J. M. Alfaro, B. Fraile, M. V. T. Lobo, M. Royuela, R. Paniagua, and M. I. Arenas
Immunohistochemical Detection of the Retinoid X Receptors {alpha}, {beta}, and {gamma} in Human Prostate
J Androl, January 1, 2003; 24(1): 113 - 119.
[Abstract] [Full Text] [PDF]

Home page
 J. Am. Soc. Nephrol.Home page
R. Ohashi, A. Shimizu, Y. Masuda, H. Kitamura, M. Ishizaki, Y. Sugisaki, and N. Yamanaka
Peritubular Capillary Regression during the Progression of Experimental Obstructive Nephropathy
J. Am. Soc. Nephrol., July 1, 2002; 13(7): 1795 - 1805.
[Abstract] [Full Text] [PDF]

Home page
 Biol. Bull.Home page
P. Compere, M.-F. Jaspar-Versali, and G. Goffinet
Glycoproteins from the Cuticle of the Atlantic Shore Crab Carcinus maenas: I. Electrophoresis and Western-Blot Analysis by Use of Lectins
Biol. Bull., February 1, 2002; 202(1): 61 - 73.
[Abstract] [Full Text] [PDF]

Home page
 JNCI J Natl Cancer InstHome page
P. L. Porter, A. Y. El-Bastawissi, M. T. Mandelson, M. G. Lin, N. Khalid, E. A. Watney, L. Cousens, D. White, S. Taplin, and E. White
Breast Tumor Characteristics as Predictors of Mammographic Detection: Comparison of Interval- and Screen-Detected Cancers
J Natl Cancer Inst, December 1, 1999; 91(23): 2020 - 2028.
[Abstract] [Full Text] [PDF]

Home page
 Infect. Immun.Home page
M. S. Tonetti, D. Cortellini, and N. P. Lang
In Situ Detection of Apoptosis at Sites of Chronic Bacterially Induced Inflammation in Human Gingiva
Infect. Immun., November 1, 1998; 66(11): 5190 - 5195.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
I. M. Bennani-Baiti, S. L. Asa, D. Song, R. Iratni, S. A. Liebhaber, and N. E. Cooke
DNase I-hypersensitive sites I and II of the human growth hormone locus control region are a major developmental activator of somatotrope gene expression
PNAS, September 1, 1998; 95(18): 10655 - 10660.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
K. Yamagata, K. Murayama, M. Okabe, K. Toshimori, T. Nakanishi, S.-i. Kashiwabara, and T. Baba
Acrosin Accelerates the Dispersal of Sperm Acrosomal Proteins during Acrosome Reaction
J. Biol. Chem., April 24, 1998; 273(17): 10470 - 10474.
[Abstract] [Full Text] [PDF]

Home page
 Ann Rheum DisHome page
K. Gaffney, J. Cookson, S. Blades, A. Coumbe, and D. Blake
Quantitative assessment of the rheumatoid synovial microvascular bed by gadolinium-DTPA enhanced magnetic resonance imaging
Ann Rheum Dis, March 1, 1998; 57(3): 152 - 157.
[Abstract] [Full Text]

Home page
 J. Histochem. Cytochem.Home page
B. G. Short, D. M. Zimmerman, and L. W. Schwartz
Automated Double Labeling of Proliferation and Apoptosis in Glutathione S-transferase-positive Hepatocytes in Rats
J. Histochem. Cytochem., September 1, 1997; 45(9): 1299 - 1306.
[Abstract] [Full Text] [PDF]

Home page
 J. Clin. Endocrinol. Metab.Home page
S. A. A. Abbass, S. L. Asa, and S. Ezzat
Altered Expression of Fibroblast Growth Factor Receptors in Human Pituitary Adenomas
J. Clin. Endocrinol. Metab., April 1, 1997; 82(4): 1160 - 1166.
[Abstract] [Full Text] [PDF]

Home page
 Arterioscler. Thromb. Vasc. Bio.Home page
T. Kanzaki, K. Tamura, K. Takahashi, Y. Saito, B. Akikusa, H. Oohashi, N. Kasayuki, M. Ueda, and N. Morisaki
In Vivo Effect of TGF-ß1 : Enhanced Intimal Thickening by Administration of TGF-ß1 in Rabbit Arteries Injured With a Balloon Catheter
Arterioscler. Thromb. Vasc. Biol., November 1, 1995; 15(11): 1951 - 1957.
[Abstract] [Full Text]

Home page
 NEJMHome page
J. T. Beck, S.-M. Hsu, J. Wijdenes, R. Bataille, B. Klein, D. Vesole, K. Hayden, S. Jagannath, and B. Barlogie
Alleviation of Systemic Manifestations of Castleman's Disease by Monoclonal Anti-Interleukin-6 Antibody
N. Engl. J. Med., March 3, 1994; 330(9): 602 - 605.
[Full Text]

Home page
 FAKE JDRHome page
T. Okiji, N. Kawashima, T. Kosaka, A. Matsumoto, C. Kobayashi, and H. Suda
An Immunohistochemical Study of the Distribution of Immunocompetent Cells, Especially Macrophages and Ia Antigen-expressing Cells of Heterogeneous Populations, in Normal Rat Molar Pulp
Journal of Dental Research, May 1, 1992; 71(5): 1196 - 1202.
[Abstract] [PDF]

Home page
 fake Adv Dent ResHome page
R.G. Quivey JR
The Use of DNA Probes in Dental Diagnosis and Therapy
Advances in Dental Research, December 1, 1987; 1(1): 99 - 108.
[Abstract] [PDF]

Home page
 FAKE JDRHome page
K.H. Ness, T.H. Morton, and B.A. Dale
Identification of Merkel Cells in Oral Epithelium Using Antikeratin and Antineuroendocrine Monoclonal Antibodies
Journal of Dental Research, June 1, 1987; 66(6): 1154 - 1158.
[Abstract] [PDF]



Guidelines | Subscriptions | About | exPRESS - Current - Archive | Business Information | Contact
The Journal of Histochemistry & Cytochemistry is owned, published, and licensed by The Histochemical Society © 1982