|
|
|
|
 |
|||
|
|||
Some fluorescent counterstains for neuroanatomical studies
LC Schmued, LW Swanson and PE Sawchenko
Methods for counterstaining neural tissue that contains fluorescent markers have been developed. Acridine orange is useful for localizing cells that are retrogradely labelled with the fluorescent tracers true blue, bisbenzimide, and nuclear yellow because at low concentrations it yields a green Nissl stain when excited with blue, but not with ultraviolet, light; since the tracers fluoresce only when exposed to ultraviolet light, they are not masked by the counterstain. In addition, counterstaining at pH 2 increases bisbenzimide fluorescence considerably. Ethidium bromide is useful for immunohistochemistry (IHC) because it yields a bright red Nissl counterstain when excited by green light, and is only faintly visible when the fluorescein marker is excited with blue light, or when ultraviolet excitation is used. Ethidium bromide is therefore a good counterstain for fluorescent retrograde tracer and for combined IHC-retrograde tracer studies as well. Certain dyes are also useful for studies of the normal morphology of neural tissue. For example, bisbenzimide and nuclear yellow at low concentrations produce a brilliant Nissl stain at pH 2, and stain only nuclei at pH 7.2. The latter procedure may be particularly useful for cell counts. Finally, neutral red, astrazone red, and safranin-O differentially stain cells amd myelinated fibers, producing fluorescence analogs of the Kluver-Barrera stain.
Volume 30,
Issue 2,
pp. 123-128,
02/01/1982
Copyright © 1982 by The Histochemical Society
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  Z. Xiang, E. E. Nesterov, J. Skoch, T. Lin, B. T. Hyman, T. M. Swager, B. J. Bacskai, and S. A. Reeves Detection of Myelination Using a Novel Histological Probe J. Histochem. Cytochem., December 1, 2005; 53(12): 1511 - 1516. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. J. De Vries, E. F. Rissman, R. B. Simerly, L.-Y. Yang, E. M. Scordalakes, C. J. Auger, A. Swain, R. Lovell-Badge, P. S. Burgoyne, and A. P. Arnold A Model System for Study of Sex Chromosome Effects on Sexually Dimorphic Neural and Behavioral Traits J. Neurosci., October 15, 2002; 22(20): 9005 - 9014. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. J. Heynen and M. F. Bear Long-Term Potentiation of Thalamocortical Transmission in the Adult Visual Cortex In Vivo J. Neurosci., December 15, 2001; 21(24): 9801 - 9813. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K M Ekelund and E Ekblad Structural, neuronal, and functional adaptive changes in atrophic rat ileum Gut, August 1, 1999; 45(2): 236 - 245. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E Ekblad, R Sjuve, A Arner, and F Sundler Enteric neuronal plasticity and a reduced number of interstitial cells of Cajal in hypertrophic rat ileum Gut, June 1, 1998; 42(6): 836 - 844. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Q. T. Nguyen, A. S. Parsadanian, W. D. Snider, and J. W. Lichtman Hyperinnervation of Neuromuscular Junctions Caused by GDNF Overexpression in Muscle Science, March 13, 1998; 279(5357): 1725 - 1729. [Abstract] [Full Text]
|
|
|
|
|
|
J. C. Stockert, C. I. Trigoso, T. Cuellar, J. L. Bella, and J. A. Lisanti A New Fluorescence Reaction in DNA Cytochemistry: Microscopic and Spectroscopic Studies on the Aromatic Diamidino Compound M&B 938 J. Histochem. Cytochem., January 1, 1997; 45(1): 97 - 106. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D M Simmons, J W Voss, H A Ingraham, J M Holloway, R S Broide, M G Rosenfeld, and L W Swanson Pituitary cell phenotypes involve cell-specific Pit-1 mRNA translation and synergistic interactions with other classes of transcription factors. Genes & Dev., May 1, 1990; 4(5): 695 - 711. [Abstract] [PDF]
|
|
| Guidelines | Subscriptions | About | exPRESS - Current - Archive | Business Information | Contact |