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Immunohistochemical identification of mast cells in paraffin- and epon- embedded tissues using platelet factor 41

ST Shaw , PC Roche, G Schmer and LK Macaulay

An immunoperoxidase method has been developed for staining heparin in the granules of mast cells. The method employs human platelet factor 4 (or anti-heparin) and a rabbit antiserum to this polypeptide. Platelet factor 4 binds to mast cell heparin and provides the basis for immunoperoxidase staining using the rabbit antiserum. Preliminary studies of mast cells in various tissues indicate that the stain is quite specific for the content of mast cell granules, presumably heparin and possibly other glycosaminoglycans.

Volume 30, Issue 2, pp. 185-188, 02/01/1982
Copyright © 1982 by The Histochemical Society


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