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A modified glyoxylic acid-formaldehyde technique for histofluorescence of catecholamine-containing neurons in cryostat sections of the insect brain

N Klemm

A simple and rapid method is described to demonstrate catecholamine- containing neurons in the insect brain by cryostat sectioning and immersion in an ice-cold SPG (sucrose-potassium phosphate-glyoxylic acid) solution. The fluorescent yield achieved can be further intensified by heating the preparations with formaldehyde vapors. The advantages of the method as compared to the original Falck-Hillarp method are speed, simplicity, and a very distinct photostabile fluorophore. The preparations can be stored for several weeks. The drawback is a lower sensitivity than the Falck-Hillarp method provides.

Volume 30, Issue 4, pp. 398-400, 04/01/1982
Copyright © 1982 by The Histochemical Society


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The Journal of Histochemistry & Cytochemistry is owned, published, and licensed by The Histochemical Society © 1982