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A method for quantitative extraction of enzymes and metabolites from tissue samples in the milligram range

D Pette and H Reichmann

A method is described for quantitative extraction of soluble enzyme activities from tissue samples in the milligram range. Mechanical disintegration of the frozen tissue is performed in a micromortar from which the powdered tissue can be quantitatively transferred for extraction in small volumes within a microcentrifuge tube.

Volume 30, Issue 4, pp. 401-402, 04/01/1982
Copyright © 1982 by The Histochemical Society


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[Abstract] [Full Text] [PDF]




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