A method for quantitative extraction of enzymes and metabolites from tissue samples in the milligram rangeD Pette and H Reichmann
A method is described for quantitative extraction of soluble enzyme activities from tissue samples in the milligram range. Mechanical disintegration of the frozen tissue is performed in a micromortar from which the powdered tissue can be quantitatively transferred for extraction in small volumes within a microcentrifuge tube.
Volume 30,
Issue 4,
pp. 401-402,
04/01/1982
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L. Stevens, C. Firinga, B. Gohlsch, B. Bastide, Y. Mounier, and D. Pette Effects of unweighting and clenbuterol on myosin light and heavy chains in fast and slow muscles of rat Am J Physiol Cell Physiol, November 1, 2000; 279(5): C1558 - C1563. [Abstract] [Full Text] [PDF] |
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