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Visualization of intracellular trafficking: use of biotinylated ligands in conjunction with avidin-gold colloids

RE Morris and CB Saelinger

A simple, sensitive method to visualize the binding and internalization of protein ligands by cells in culture is described. A biotinylated toxin was used as ligand, and succinoylated avidin adsorbed onto 5.2 nm gold sols was the electron-dense marker. This method affords direct localization of proteins that are on the cell surface or intracellular without need for techniques that alter membrane integrity.

Volume 32, Issue 1, pp. 124-128, 01/01/1984
Copyright © 1984 by The Histochemical Society


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The Journal of Histochemistry & Cytochemistry is owned, published, and licensed by The Histochemical Society © 1984