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Cytochemical detection of hyaluronidase activity in single human and mouse sperm by an improved substrate-film technique

R Waibel, LC Ginsberg and G Ficsor

A substrate-film method is described that allows the detection of hyaluronidase activity in nearly 100% of single human and mouse sperm. The level of hyaluronidase activity as determined by halo diameters was greater in mouse than in human sperm. This simple method may have use as a screening method for identifying compounds that cause developmental or genetic defects in male germ cells, or for the diagnosis of infertility due to decreased hyaluronidase activity.

Volume 32, Issue 1, pp. 63-66, 01/01/1984
Copyright © 1984 by The Histochemical Society


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The Journal of Histochemistry & Cytochemistry is owned, published, and licensed by The Histochemical Society © 1984