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Visualization of peptide-immunoreactive processes on serotonin- immunoreactive cells using two-color immunoperoxidase staining

MB Hancock

The sequential application of the peroxidase-antiperoxidase (PAP) technique with nickel-intensified DAB and with DAB alone was used to visualize black peptide-immunoreactive endings on amber serotonin- immunoreactive cells in 1-2-micron paraffin sections of the hamster medulla. Met-enkephalin- and substance P-positive terminals were present on serotonin cells in the raphe nuclei, the ventral reticular formation, and in the nucleus interfascicularis hypoglossi. The presence of enkephalin-immunoreactive endings on medullary serotonin- immunoreactive cells correlates with the analgesia and autonomic changes that result from the application of morphine or met-enkephalin to the medulla.

Volume 32, Issue 3, pp. 311-314, 03/01/1984
Copyright © 1984 by The Histochemical Society


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