Journal of Histochemistry and Cytochemistry Priciples for Free Access to Science
  Search:   
    >> Advanced Search

Guidelines | Subscriptions | About | exPRESS - Current - Archive | Business Information | Contact
This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Warhol, M. J.
Right arrow Articles by Rice, R. H.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Warhol, M. J.
Right arrow Articles by Rice, R. H.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?

Immuno-ultrastructural localization of involucrin in squamous epithelium and cultured keratinocytes

MJ Warhol, J Roth, JM Lucocq, GS Pinkus and RH Rice

Involucrin immunoreactivity was localized ultrastructurally with protein A--gold in epidermis and cultured keratinocytes embedded in Lowicryl K4M. In the skin, immunoreactivity was found predominantly in cells of the granular layer and inner stratum corneum. The label was associated primarily with amorphous cytoplasmic material and especially keratohyaline granules. Some labeling was observed at the cell periphery, but little with keratin filaments. Tissue samples examined without aldehyde fixation showed relatively greater labeling in the outer stratum corneum than fixed tissue. In cultured cells, the labeling was also associated primarily with cytoplasmic granular material and to a lesser extent with the cell periphery. Upon treatment with the ionophore X537A, keratin filaments were found in aggregated arrays and the plasma membranes became convoluted. That involucrin immunoreactivity persisted in the cytoplasm in cultured cells and in vivo after cross-linking occurs could account for considerable isopeptide bonding detected in epidermal keratin fractions and indicates that not all the involucrin participates in envelope formation.

Volume 33, Issue 2, pp. 141-149, 02/01/1985
Copyright © 1985 by The Histochemical Society


Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:


Home page
 J. Histochem. Cytochem.Home page
L. Alibardi, M. Dockal, C. Reinisch, E. Tschachler, and L. Eckhart
Ultrastructural Localization of Caspase-14 in Human Epidermis
J. Histochem. Cytochem., December 1, 2004; 52(12): 1561 - 1574.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
J. F. Crish, T. M. Zaim, and R. L. Eckert
The Distal Regulatory Region of the Human Involucrin Promoter Is Required for Expression in Epidermis
J. Biol. Chem., November 13, 1998; 273(46): 30460 - 30465.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
T. Efimova, P. LaCelle, J. F. Welter, and R. L. Eckert
Regulation of Human Involucrin Promoter Activity by a Protein Kinase C, Ras, MEKK1, MEK3, p38/RK, AP1 Signal Transduction Pathway
J. Biol. Chem., September 18, 1998; 273(38): 24387 - 24395.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
P. M. Steinert and L. N. Marekov
Direct Evidence That Involucrin Is a Major Early Isopeptide Cross-linked Component of the Keratinocyte Cornified Cell Envelope
J. Biol. Chem., January 17, 1997; 272(3): 2021 - 2030.
[Abstract] [Full Text] [PDF]



Guidelines | Subscriptions | About | exPRESS - Current - Archive | Business Information | Contact
The Journal of Histochemistry & Cytochemistry is owned, published, and licensed by The Histochemical Society © 1985