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The use of monoclonal antibodies to estrogen receptors (ER) for immunoperoxidase detection of ER in paraffin sections of human breast cancer tissue

HS Poulsen, L Ozzello, WJ King and GL Greene

Two monoclonal antibodies against MCF-7 human estrogen receptors were used for immunoperoxidase staining of paraffin sections of human breast cancer tissue. The staining was predominantly located in the nucleus of epithelial cells. Variation in the staining intensity was observed among individual cells. A significant positive correlation between the number of positively stained cells and cytosol estrogen receptor content (fmol of bound estrogen/mg of protein) was observed. The potential and the limitations of the present techniques are discussed.

Volume 33, Issue 2, pp. 87-92, 02/01/1985
Copyright © 1985 by The Histochemical Society


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S. H. Schurman, N. Sharon, R. A. Goldschmidt, and E. F. Scanlon
Improved Detection of Metastases to Lymph Nodes and Estrogen Receptor Determination
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[Abstract] [PDF]

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D. C. Allred, M. A. Bustamante, C. O. Daniel, H. V. Gaskill, and A. B. Cruz Jr
Immunocytochemical Analysis of Estrogen Receptors in Human Breast Carcinomas: Evaluation of 130 Cases and Review of the Literature Regarding Concordance With Biochemical Assay and Clinical Relevance
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[Abstract] [PDF]



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