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Improved localization of intracellular sites of phosphatases using cerium and cell permeabilization

JM Robinson

A simple permeabilization method has been developed that allows for intracellular localization of acid phosphatase in neutrophils and several types of tissue culture cells with cerium. This permeabilization procedure also facilitates intracellular alkaline phosphatase localization in neutrophils without the loss of cell surface reaction in this cell type. Only the cell surface reaction was detected in the absence of permeabilization. Glutaraldehyde-fixed cells were permeabilized with detergent during the cytochemical reaction. Triton X-100 at 0.0001-0.0002% gave the best results for the enzymes and cell types tested.

Volume 33, Issue 8, pp. 749-754, 08/01/1985
Copyright © 1985 by The Histochemical Society


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