Journal of Histochemistry and Cytochemistry Priciples for Free Access to Science
  Search:   
    >> Advanced Search

Guidelines | Subscriptions | About | exPRESS - Current - Archive | Business Information | Contact
This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Press, M. F.
Right arrow Articles by Greene, G. L.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Press, M. F.
Right arrow Articles by Greene, G. L.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?

Immunoelectron microscopic localization of estrogen receptor with monoclonal estrophilin antibodies

MF Press, NA Nousek-Goebl and GL Greene

The recent production of a series of monoclonal estrophilin (estrogen receptor) antibodies recognizing estrogen receptor derived from a wide variety of animals and target tissues permits the development of immunoelectron microscopic techniques for identifying estrogen receptor. We have determined suitable conditions for the ultrastructural localization of estrogen receptor in tissue sections. Localization of receptor was observed in the euchromatin, but not in the marginated heterochromatin or nucleoli of epithelial and stromal nuclei of human endometrium. Competition studies indicate that only estrogen receptor specifically inhibits nuclear staining. The absence of any specific cytoplasmic localization at the electron-microscopic level is consistent with earlier light-microscopic observations and suggests that the majority of the cellular pool of estrophilin exists in the nucleus of hormone-responsive cells.

Volume 33, Issue 9, pp. 915-924, 09/01/1985
Copyright © 1985 by The Histochemical Society


Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:


Home page
 Mol. Biol. CellHome page
H. Htun, L. T. Holth, D. Walker, J. R. Davie, and G. L. Hager
Direct Visualization of the Human Estrogen Receptor alpha  Reveals a Role for Ligand in the Nuclear Distribution of the Receptor
Mol. Biol. Cell, February 1, 1999; 10(2): 471 - 486.
[Abstract] [Full Text]

Home page
 J. Biol. Chem.Home page
J. P. Aumais, H. S. Lee, R. Lin, and J. H. White
Selective Interaction of hsp90 with an Estrogen Receptor Ligand-binding Domain Containing a Point Mutation
J. Biol. Chem., May 2, 1997; 272(18): 12229 - 12235.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
H. S. Lee, J. Aumais, and J. H. White
Hormone-dependent Transactivation by Estrogen Receptor Chimeras That Do Not Interact with hsp90. EVIDENCE FOR TRANSCRIPTIONAL REPRESSORS
J. Biol. Chem., October 18, 1996; 271(42): 25727 - 25730.
[Abstract] [Full Text] [PDF]

Home page
 ScienceHome page
G. Greene, P Gilna, M Waterfield, A Baker, Y Hort, and J Shine
Sequence and expression of human estrogen receptor complementary DNA
Science, March 7, 1986; 231(4742): 1150 - 1154.
[Abstract] [PDF]



Guidelines | Subscriptions | About | exPRESS - Current - Archive | Business Information | Contact
The Journal of Histochemistry & Cytochemistry is owned, published, and licensed by The Histochemical Society © 1985