A fluorescence staining method for the demonstration and measurement of lysosomal enzyme activities in single cellsGP Luyten, AT Hoogeveen and H Galjaard
A cytochemical fluorescence method is described that makes possible simple, rapid, and specific demonstration and measurement of the activities of a wide variety of lysosomal enzymes in single cells using 4-methylumbelliferyl derivatives as substrates. The validity of the method and a number of applications using normal and mutant human cells are presented.
Volume 33,
Issue 9,
pp. 965-968,
09/01/1985
|
|||||||||||||
| Guidelines | Subscriptions | About | exPRESS - Current - Archive | Business Information | Contact |