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A fluorescence staining method for the demonstration and measurement of lysosomal enzyme activities in single cells

GP Luyten, AT Hoogeveen and H Galjaard

A cytochemical fluorescence method is described that makes possible simple, rapid, and specific demonstration and measurement of the activities of a wide variety of lysosomal enzymes in single cells using 4-methylumbelliferyl derivatives as substrates. The validity of the method and a number of applications using normal and mutant human cells are presented.

Volume 33, Issue 9, pp. 965-968, 09/01/1985
Copyright © 1985 by The Histochemical Society


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The Journal of Histochemistry & Cytochemistry is owned, published, and licensed by The Histochemical Society © 1985