Journal of Histochemistry and Cytochemistry Priciples for Free Access to Science
  Search:   
    >> Advanced Search

Guidelines | Subscriptions | About | exPRESS - Current - Archive | Business Information | Contact
This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Jones, K. H.
Right arrow Articles by Kniss, D. A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Jones, K. H.
Right arrow Articles by Kniss, D. A.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?

Propidium iodide as a nuclear counterstain for immunofluorescence studies on cells in culture

KH Jones and DA Kniss

We describe a rapid procedure using propidium iodide (PI) as a nuclear counterstain in immunofluorescence studies where cell surface or intracellular antigens are localized with fluorescein-conjugated antisera. In fixed monolayer preparations, all cell nuclei fluoresce red and can be seen simultaneously with cellular antigens that fluoresce green. Counterstaining with PI therefore makes possible quantification of the proportion of cells present in culture that stain immunocytochemically for a specific antigen.

Volume 35, Issue 1, pp. 123-125, 01/01/1987
Copyright © 1987 by The Histochemical Society


Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:


Home page
 Ann. Thorac. Surg.Home page
U. Sprengel, B. Munkel, F. Robicsek, T. N. Masters, and J. Schaper
Ischemia Affects Cardiac Proteins in Healthy Animals Less Severely Than in Human Patients
Ann. Thorac. Surg., September 1, 1995; 60(3): 767 - 772.
[Abstract] [Full Text]

Home page
 J. Thorac. Cardiovasc. Surg.Home page
S. Hein, T. Scheffold, and J. Schaper
Ischemia induces early changes to cytoskeletal and contractile proteins in diseased human myocardium
J. Thorac. Cardiovasc. Surg., July 1, 1995; 110(1): 89 - 98.
[Abstract] [Full Text]



Guidelines | Subscriptions | About | exPRESS - Current - Archive | Business Information | Contact
The Journal of Histochemistry & Cytochemistry is owned, published, and licensed by The Histochemical Society © 1987