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Resolution in EM autoradiography V: 45Ca (and 33P)

MM Salpeter, R Nelson, R Harris and EE Salpeter

We devised a specimen to test EM autoradiographic resolution for calcium-45 (and phosphorous-33). A dry emulsion film was used for soluble compounds. We obtained a resolution (HD) value of 2300 +/- 500 A. This value is no larger than that previously obtained for Carbon - 14, whose emission is about 1.6-fold lower. This result is as expected on theoretical grounds.

Volume 35, Issue 10, pp. 1047-1051, 10/01/1987
Copyright © 1987 by The Histochemical Society


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The Journal of Histochemistry & Cytochemistry is owned, published, and licensed by The Histochemical Society © 1987