|
|
|
|
 |
|||
|
|||
Identification of tissue histiocytes on paraffin sections by a new monoclonal antibody
DJ Flavell, DB Jones and DH Wright
University Department of Pathology, Southampton General Hospital, United Kingdom.
A mouse monoclonal antibody (MAC 387) with specificity for monocytes and tissue histiocytes was produced by immunization of a BALB/c mouse with peripheral blood monocyte components derived by affinity chromatography of detergent-solubilized monocyte material on Sepharose 4B coupled to rabbit anti-monocyte antibodies. MAC 387 strongly stained the cytoplasm of cells of the monocyte/macrophage series on paraffin sections after controlled trypsinization of sections. The antibody showed broad reactivity for a variety of tissue histiocytes, including infiltrating and reactive histiocytes, alveolar macrophages, Kupffer cells, follicle-center macrophages, splenic red pulp macrophages, tumor- infiltrating macrophages, sinus histiocytes, epithelioid giant cells (variably), and cases of histiocytosis X and dermatopathic lymphadenopathy. Molecular weight data obtained by Western blotting, immunoprecipitation, and immunoaffinity-purification revealed that the antigen was present in different forms in the monocyte and granulocyte. In the granulocyte, free alpha (Mr 12 KD) and beta (Mr 14 KD) chains expressing the MAC 387 epitope were found together with associations of one alpha and one beta chain linked by disulfide bonds to yield a heterodimer of Mr 26 KD. In the monocyte, free alpha and beta chains are not found, but instead the heterodimer and associations of two (Mr 56 KD) and four (Mr 112 KD) heterodimers are disulfide-linked together. This new monoclonal reagent should have particular value for identification of tissue histiocytes in routine paraffin sections and particularly for demonstration of histiocytes in malignant lymphomas.
Volume 35,
Issue 11,
pp. 1217-1226,
11/01/1987
Copyright © 1987 by The Histochemical Society
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  J. Schwaller, P. Schneider, P. Mhawech-Fauceglia, T. McKee, S. Myit, T. Matthes, J. Tschopp, O. Donze, F.-A. Le Gal, and B. Huard Neutrophil-derived APRIL concentrated in tumor lesions by proteoglycans correlates with human B-cell lymphoma aggressiveness Blood, January 1, 2007; 109(1): 331 - 338. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 V. Roulet, A.-P. Satie, A. Ruffault, A. L. Tortorec, H. Denis, O. Guist'hau, J.-J. Patard, N. Rioux-Leclerq, J. Gicquel, B. Jegou, et al. Susceptibility of Human Testis to Human Immunodeficiency Virus-1 Infection in Situ and in Vitro Am. J. Pathol., December 1, 2006; 169(6): 2094 - 2103. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 V. Roulet, H. Denis, C. Staub, A. Le Tortorec, B. Delaleu, A.P. Satie, J.J. Patard, B. Jegou, and N. Dejucq-Rainsford Human testis in organotypic culture: application for basic or clinical research Hum. Reprod., June 1, 2006; 21(6): 1564 - 1575. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. Habasque, F. Aubry, B. Jegou, and M. Samson Study of the HIV-1 receptors CD4, CXCR4, CCR5 and CCR3 in the human and rat testis Mol. Hum. Reprod., May 1, 2002; 8(5): 419 - 425. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Kipar, J. Kremendahl, M. L. Jackson, and M. Reinacher Comparative Examination of Cats with Feline Leukemia Virus-associated Enteritis and Other Relevant Forms of Feline Enteritis Vet. Pathol., July 1, 2001; 38(4): 359 - 371. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P H McGuinness, D Painter, S Davies, and G W McCaughan Increases in intrahepatic CD68 positive cells, MAC387 positive cells, and proinflammatory cytokines (particularly interleukin 18) in chronic hepatitis C infection Gut, February 1, 2000; 46(2): 260 - 269. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Christgau, R. G. Caffesse, J. R. Newland, G. Schmalz, and R. N. D'Souza Characterization of Immunocompetent Cells in the Diseased Canine Periodontium J. Histochem. Cytochem., December 1, 1998; 46(12): 1443 - 1454. [Abstract] [Full Text]
|
|
|
|
 |
|
 M. Jontell, T. Okiji, U. Dahlgren, and G. Bergenholtz Immune Defense Mechanisms of the Dental Pulp Critical Reviews in Oral Biology & Medicine, January 1, 1998; 9(2): 179 - 200. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 F. A Stockl, A. M Dolmetsch, M A. Saornil, R. L Font, and M. N Burnier Jr Orbital granulocytic sarcoma Br. J. Ophthalmol., December 1, 1997; 81(12): 1084 - 1088. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K.T. Miyasaki, A.L. Bodeau, A.R.K. Murthy, and R.I. Lehrer In vitro Antimicrobial Activity of the Human Neutrophil Cytosolic S-100 Protein Complex, Calprotectin, Against Capnocytophaga sputigena Journal of Dental Research, February 1, 1993; 72(2): 517 - 523. [PDF]
|
|
| Guidelines | Subscriptions | About | exPRESS - Current - Archive | Business Information | Contact |