|
|
|
|
 |
|||
|
|||
Immunocytochemical localization of P170 at the plasma membrane of multidrug-resistant human cells
MC Willingham, ND Richert, MM Cornwell, T Tsuruo, H Hamada, MM Gottesman and IH Pastan
Laboratory of Molecular Biology, National Cancer Institute, Bethesda, MD 20892.
P170 (P-glycoprotein) is a membrane protein found in high levels in multidrug-resistant cultured cell lines. We have localized this protein using monoclonal antibody MRK16 by immunofluorescence and electron microscopy in the multidrug-resistant human carcinoma cell line KB-C4. The P170 determinant recognized by antibody MRK16 was found on drug- resistant KB-C4 cells, but not on parental drug-sensitive KB-3-1 cells. The determinant was present on the external surface of the plasma membrane and on the luminal side of Golgi stack membranes. P170 was excluded from coated pits at the plasma membrane and absent from endocytic vesicles and lysosomes. This determinant was detected only in small amounts in the endoplasmic reticulum. The high protein concentration of P170 in the plasma membrane is consistent with a role of this protein as a drug efflux pump at the cell surface.
Volume 35,
Issue 12,
pp. 1451-1456,
12/01/1987
Copyright © 1987 by The Histochemical Society
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  D. Fu and B. D. Roufogalis Actin disruption inhibits endosomal traffic of P-glycoprotein-EGFP and resistance to daunorubicin accumulation Am J Physiol Cell Physiol, April 1, 2007; 292(4): C1543 - C1552. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. R. Johnson, R. A. Finch, Z. P. Lin, C. J. Zeiss, and A. C. Sartorelli The Pharmacological Phenotype of Combined Multidrug-Resistance mdr1a/1b- and mrp1-deficient Mice Cancer Res., February 1, 2001; 61(4): 1469 - 1476. [Abstract] [Full Text]
|
|
|
|
 |
|
 Y. RAVIV, A. PURI, and R. BLUMENTHAL P-glycoprotein-overexpressing multidrug-resistant cells are resistant to infection by enveloped viruses that enter via the plasma membrane FASEB J, March 1, 2000; 14(3): 511 - 515. [Abstract] [Full Text]
|
|
|
|
 |
|
 P. Lala, S. Ito, and C. A. Lingwood Retroviral Transfection of Madin-Darby Canine Kidney Cells with Human MDR1 Results in a Major Increase in Globotriaosylceramide and 105- to 106-Fold Increased Cell Sensitivity to Verocytotoxin. ROLE OF P-GLYCOPROTEIN IN GLYCOLIPID SYNTHESIS J. Biol. Chem., February 25, 2000; 275(9): 6246 - 6251. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. Millot, J.-M. Millot, H. Morjani, A. Desplaces, and M. Manfait Characterization of Acidic Vesicles in Multidrug-resistant and Sensitive Cancer Cells by Acridine Orange Staining and Confocal Microspectrofluorometry J. Histochem. Cytochem., September 1, 1997; 45(9): 1255 - 1264. [Abstract] [Full Text] [PDF]
|
|
| Guidelines | Subscriptions | About | exPRESS - Current - Archive | Business Information | Contact |