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Use of azide and hydrogen peroxide as an inhibitor for endogenous peroxidase in the immunoperoxidase method

CY Li, SC Ziesmer and O Lazcano-Villareal

Department of Laboratory Medicine, Mayo Clinic, Rochester, Minnesota 55905.

We describe a simple and effective method for inhibition of endogenous peroxidase activity in the immunoperoxidase technique. Specimens are pre-treated with a mixture of azide and hydrogen peroxide, which is then followed by an indirect immunoperoxidase procedure. Comparison studies showed no significant loss of antigenicity or morphological details by this pre-treatment. The method is most useful for evaluating cell-specific antigens on specimens that have abundant endogenous peroxidase activity, such as blood and bone marrow.

Volume 35, Issue 12, pp. 1457-1460, 12/01/1987
Copyright © 1987 by The Histochemical Society


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The Journal of Histochemistry & Cytochemistry is owned, published, and licensed by The Histochemical Society © 1987