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Cytochemical detection of carbohydrate and immunocytochemical detection of relaxin in the same secretory granule

LH Larkin, DE Welch, S Ogilvie and L Wubbel

We administered estradiol and progesterone to spayed guinea pigs, with resultant accumulation of secretory granules in endometrial gland cells. By initially employing protein A-colloidal gold immunolocalization of relaxin, followed by cytochemical staining of carbohydrate with the thiocarbohydrazide-silver proteinate method on the same section, we showed clearly that the secretory granules were composed of a central core containing relaxin and a cortex of carbohydrate-rich material. Use of normal rabbit serum rather than relaxin antiserum, and omission of periodic acid, demonstrated the specificity of the technique.

Volume 35, Issue 6, pp. 693-697, 06/01/1987
Copyright © 1987 by The Histochemical Society


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