|
|
|
|
 |
|||
|
|||
Ultrastructural localization of rat Clara cell 10 KD secretory protein by the immunogold technique using polyclonal and monoclonal antibodies
CD Bedetti, J Singh, G Singh, SL Katyal and ML Wong-Chong
Using a monoclonal antibody and affinity-purified polyclonal antiserum against a 10 KD protein isolated from rat pulmonary lavage, we have localized the protein within Clara cells by a post-embedment protein A- gold technique. The gold particles were localized over the secretory granules of rat Clara cells. Ultrastructural immunolocalization was abolished when the primary antibodies were previously absorbed with purified 10 KD protein. Other pulmonary cells, including type II pneumocytes and ciliated cells, were negative with this technique. These results demonstrate the presence of the 10 KD protein in the secretory granules of the Clara cell and support the concept that this protein constitutes a specific and unique secretory product of Clara cells.
Volume 35,
Issue 7,
pp. 789-794,
07/01/1987
Copyright © 1987 by The Histochemical Society
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  F. Merigo, D. Benati, M. Galie, C. Crescimanno, F. Osculati, and A. Sbarbati Immunohistochemical Localization of Cystic Fibrosis Transmembrane Regulator and Clara Cell Secretory Protein in Taste Receptor Cells of Rat Circumvallate Papillae Chem Senses, March 1, 2008; 33(3): 231 - 241. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. J. Homer, Z. Zhu, L. Cohn, C. G. Lee, W. I. White, S. Chen, and J. A. Elias Differential expression of chitinases identify subsets of murine airway epithelial cells in allergic inflammation Am J Physiol Lung Cell Mol Physiol, September 1, 2006; 291(3): L502 - L511. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. D. Reynolds, P. R. Reynolds, G. S. Pryhuber, J. D. Finder, and B. R. Stripp Secretoglobins SCGB3A1 and SCGB3A2 Define Secretory Cell Subsets in Mouse and Human Airways Am. J. Respir. Crit. Care Med., December 1, 2002; 166(11): 1498 - 1509. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. E. BOERS, A. W. AMBERGEN, and F. B. J. M. THUNNISSEN Number and Proliferation of Clara Cells in Normal Human Airway Epithelium Am. J. Respir. Crit. Care Med., May 1, 1999; 159(5): 1585 - 1591. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. HERMANS and A. BERNARD Lung Epithelium-specific Proteins . Characteristics and Potential Applications as Markers Am. J. Respir. Crit. Care Med., February 1, 1999; 159(2): 646 - 678. [Full Text]
|
|
|
|
|
|
C.-M. Ji, F. H. Royce, U. Truong, C. G. Plopper, G. Singh, and K. E. Pinkerton Maternal exposure to environmental tobacco smoke alters Clara cell secretory protein expression in fetal rat lung Am J Physiol Lung Cell Mol Physiol, November 1, 1998; 275(5): L870 - L876. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Jain-Vora, S. E. Wert, U.-A. Temann, J. A. Rankin, and J. A. Whitsett Interleukin-4 Alters Epithelial Cell Differentiation and Surfactant Homeostasis in the Postnatal Mouse Lung Am. J. Respir. Cell Mol. Biol., November 1, 1997; 17(5): 541 - 551. [Abstract] [Full Text]
|
|
|
|
|
|
C. J. Johnston, G. W. Mango, J. N. Finkelstein, and B. R. Stripp Altered Pulmonary Response to Hyperoxia in Clara Cell Secretory Protein Deficient Mice Am. J. Respir. Cell Mol. Biol., August 1, 1997; 17(2): 147 - 155. [Abstract] [Full Text]
|
|
| Guidelines | Subscriptions | About | exPRESS - Current - Archive | Business Information | Contact |