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Label-fracture of cell surfaces by replica staining

CA Forsman and P Pinto da Silva

Membrane Biology Section/Laboratory of Mathematical Biology, National Cancer Institute-Frederick Cancer Research Facility, Maryland 21701.

We introduce replica-staining label-fracture, a method for the cytochemical mapping of membrane surfaces. This method is a corollary of the rationale of label-fracture (Pinto da Silva and Kan, 1984: J Cell Biol 99:1156). After freeze-fracture the exoplasmic halves of the membrane remain attached to the replica. We show that cytochemical labeling of cell surfaces can be performed by direct post-fracture staining of freeze-fracture replicas. This new variant of label- fracture leads to miniaturization of labeling procedures and allows standardization of labeling conditions and simultaneous processing of different specimens.

Volume 36, Issue 11, pp. 1413-1418, 11/01/1988
Copyright © 1988 by The Histochemical Society


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K Fujimoto
Freeze-fracture replica electron microscopy combined with SDS digestion for cytochemical labeling of integral membrane proteins. Application to the immunogold labeling of intercellular junctional complexes
J. Cell Sci., January 11, 1995; 108(11): 3443 - 3449.
[Abstract] [PDF]



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