Analysis of commercial samples of acridine orange using high- performance liquid chromatographyRD Paulsen and GS Nettleton Department of Anatomical Sciences and Neurobiology, University of Louisville, Kentucky 40292. We analyzed commercial samples of acridine orange using reverse-phase high-performance liquid chromatography. The mobile phase of 90:10:2.5 acetonitrile:de-ionized water:pentane sulfonic acid gave baseline separations of components of acridine orange samples in 15 min. Many of the samples were fairly homogeneous; the absorbance due to the acridine orange component ranged from 83-97%. Among the 11 samples tested, one was not acridine orange, reputedly different samples were identical, and the principal component of one sample was not acridine orange.
Volume 36,
Issue 12,
pp. 1489-1493,
12/01/1988
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