Journal of Histochemistry and Cytochemistry Priciples for Free Access to Science
  Search:   
    >> Advanced Search

Guidelines | Subscriptions | About | exPRESS - Current - Archive | Business Information | Contact
This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Moore, P. B.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Moore, P. B.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?

Isolation and purification of an antibody to 67-KD calcimedin

PB Moore

Laboratory Animal Research Center, Rockefeller University, New York, New York 10021-6399.

The 67-KD calcimedin is a calcium-binding protein isolated from several muscle tissues. The protein shows apparent Mr of 67,000 by SDS- polyacrylamide gel electrophoresis. An antibody has been prepared by immunizing sheep with the protein purified from chicken gizzard smooth muscle. This antibody recognizes 67-KD calcimedin but not calmodulin, bovine serum albumin, transferrin, or brain p68 calelectrin. The presence of 67-KD calcimedin is demonstrated in the smooth muscle cell lines A10 and DDT1MF-2 as well as in primary cultures of chicken breast and heart muscle, by immunoprecipitation and immunofluorescence. The 67- KD calcimedin, being responsive to calcium, may play a role in calcium- mediated cell regulation. This report identifies several cells that may be useful for further delineation of the cellular role of 67-KD calcimedin.

Volume 36, Issue 2, pp. 185-192, 02/01/1988
Copyright © 1988 by The Histochemical Society


Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?





Guidelines | Subscriptions | About | exPRESS - Current - Archive | Business Information | Contact
The Journal of Histochemistry & Cytochemistry is owned, published, and licensed by The Histochemical Society © 1988