|
|
|
|
 |
|||
|
|||
Immunogold quantitation of laminin, type IV collagen, and heparan sulfate proteoglycan in a variety of basement membranes
DS Grant and CP Leblond
Department of Anatomy, McGill University, Montreal, Quebec, Canada.
A series of basement membranes was immunolabeled for laminin, type IV collagen, and heparan sulfate proteoglycan in the hope of comparing the content of these substances. The basement membranes, including thin ones (less than 0.3 micron) from kidney, colon, enamel organ, and vas deferens, and thick ones (greater than 2 micron), i.e., Reichert's membrane, Descemet's membrane, and EHS tumor matrix, were fixed in formaldehyde, embedded in Lowicryl, and treated with specific antisera or antibodies followed by anti-rabbit immunoglobulin bound to gold. The density of gold particles, expressed per micron2, was negligible in controls (less than or equal to 1.1), but averaged 307, 146, and 23, respectively, for laminin, collagen IV, and proteoglycan over the thick basement membranes (except for Descemet's membranes, over which the density was 16, 5, and 34, respectively) and 117, 72, and 64, respectively, over the lamina densa of the thin basement membranes. Lower but significant reactions were observed over the lamina lucida. Interpretation of the gold particle densities was based on (a) the similarity between the ultrastructure of most thick basement membranes and of the lamina densa of most thin basement membranes, and (b) the biochemical content of the three substances under study in the EHS tumor matrix (Eur J Biochem 143:145, 1984). It was proposed that thick basement membranes (except Descemet's) contained more laminin and collagen IV but less heparan sulfate proteoglycan than the lamina densa of thin basement membranes. In the latter, there was a fair variation from tissue to tissue, but a tendency towards a similar molar content of the three substances.
Volume 36,
Issue 3,
pp. 271-283,
03/01/1988
Copyright © 1988 by The Histochemical Society
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  J. D. Gottsch, C. Zhang, O. H. Sundin, W. R. Bell, W. J. Stark, and W. R. Green Fuchs Corneal Dystrophy: Aberrant Collagen Distribution in an L450W Mutant of the COL8A2 Gene Invest. Ophthalmol. Vis. Sci., December 1, 2005; 46(12): 4504 - 4511. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J A P Gomes, R Amankwah, A Powell-Richards, and H S Dua Sodium hyaluronate (hyaluronic acid) promotes migration of human corneal epithelial cells in vitro Br. J. Ophthalmol., June 1, 2004; 88(6): 821 - 825. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 N. Miosge, F. Quondamatteo, C. Klenczar, and R. Herken Nidogen-1: Expression and Ultrastructural Localization During the Onset of Mesoderm Formation in the Early Mouse Embryo J. Histochem. Cytochem., February 1, 2000; 48(2): 229 - 238. [Abstract] [Full Text]
|
|
|
|
 |
|
 S. Ulisse, N. Rucci, D. Piersanti, E. Carosa, F. M. Graziano, A. Pavan, P. Ceddia, M. Arizzi, P. Muzi, L. Cironi, et al. Regulation by Thyroid Hormone of the Expression of Basement Membrane Components in Rat Prepubertal Sertoli Cells Endocrinology, February 1, 1998; 139(2): 741 - 747. [Abstract] [Full Text] [PDF]
|
|
| Guidelines | Subscriptions | About | exPRESS - Current - Archive | Business Information | Contact |